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SPECIES: mouse, BALB/c
DSMZ catalog code: ACC 51
Morphology: single, round cells in suspension
TRANSFORMED BY: Moloney-MuLV
Description: mouse lymphoma established from a BALB/c mouse induced by MOLV; described to be growth-dependent on IL-3, but to recognize also GM-CSF confirmed as mouse with IEF of AST, G6PD, NP, PEP B
Viruses: ELISA: reverse transcriptase negative
Depositor: Dr. E. Schmitt, Institute of Immunology, University of Mainz, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
95% Iscove's MDM + 5% FBS + 2 mM L-glutamine + 2.5 x 10-5 M mercaptoethanol + IL-3 (either 30% vol conditioned medium of WEHI-3B cell line (DSM ACC 26) or 20 U/ml mIL-3) maintain at 0.2-0.4 x 106 cells/ml; split 1:2 to 1:3 every 1-2 days, cells grow fast at 37C with 5% CO2 maximal density at 0.8 x 106 cells/ml;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule;
negative in DAPI, microbiological culture assays;
Validated by: isoenzymes (confirmed as mouse with IEF of AST, G6PD, NP, PEP B)
- Cell 1985;41:685-693 - PMID: 2988783
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.