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SPECIES: mouse, BALB/c
ECACC catalog code: 90110506
Description: Mouse BALB/c myeloma
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Products: Immunoglobulin G (IgG2b) ( kappa) Applications: Fusion partner, immunoglobulin production
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Maintain cultures between 4-5x100,000 cells/ml; 5% CO2; 37C. Cells achieve a maximum cell density of 1-1.5x1,000,000 cells/ml.
Derived from a female BALB/c myeloma tumour MPC-11. The parental cell line has mutagenised and grown in 6-thioguanine to establish the drug resistant line 45.6.TG1.7. This line is HPRT negative and produces mouse IgG2b kappa immunoglobulins.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 1608
- Cell 1976;8:405 - PMID: 821618
- J Exp Med 1970;131:515 - PMID: 4189836
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.