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SPECIES: fish - trout, rainbow
DSMZ catalog code: ACC 77
Morphology: adherent, fibroblastoid cells growing as monolayers
Description: fish rainbow trout liver this cell line is a subclone of the original cell line R1 established after elimination of mycoplasma with BM-Cyclin (tiamulin & minocycline)
Depositor: Dr. M. Kohlpoth, Akademie fuer Tierschutz, Neubiberg, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% Medium 199 (25 mM HEPES) with Earle's salts + 10% FBS + 2 mM L-glutamine split confluent culture 1:2 every 4-7 days using trypsin/EDTA in PBS without Ca 2+ /Mg 2+ at +4C for 5-10 min at room temperature (about 20C) without CO2 (keep lid of flask closed), cells can be stored temporarily (up to 1 week) at 4C about 5-15 x 106 cells/80 cm 2;
doubling time of about 40 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at 2 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
- Zbl Bakt Hyg B 1985;180:480-504
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.