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DSMZ catalog code: ACC 129
Morphology: small circular cells, adherent, grow to large colonies in which the single cells are no longer discernible
Description: human colon adenocarcinoma established from the tumor tissue of a 44-year-old woman with well-differentiated colon adenocarcinoma in 1984 confirmed as human with IEF of AST, MDH
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: obtained from DKFZ Tumorbank (TZB No. 610004), Heidelberg, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% Dulbecco's MEM + 10% FBS split confluent culture 1:3 to 1:6 every 3-5 days using trypsin/EDTA, seed out about 10 6 cells/80 cm 2 at 37C with 5% CO2 about 30 x 106 cells/175 cm 2;
doubling time of ca. 30 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (hypertriploid karyotype with 14% polyploidy; 72(65-74)<3n>XX, -X, +4, +7, -8, -8, -9, +11, +11, -13, +15, +17, -18, +2mar, der(4)t(1;4)(p11;p15)t(1;?)(p32-36;?), del(4)(q32), del(7)(p21), i(13q), add(15)(p11), del(17)(p12),a) * cytogenetics 2 (two small markers present; rearrangements of ch1, 7, 17 typical of colon carcinoma) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (cytokeratin+ desmin- endothel- GFAP- neurofil- vimentin-) * isoenzymes (confirmed as human with IEF of AST, MDH) * reverse transcriptase (reverse transcriptase negative)
- Eur J Cancer 1981;17:129-142 - PMID: 6894902
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.