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TISSUE/ORGAN: peripheral blood
TUMOR: leukemia, acute lymphoblastic T cell
DSMZ catalog code: ACC 40
Morphology: round or multiformed cells, singly or clustered in suspension
Description: human acute myeloid leukemia established from the peripheral blood of a 40-year-old female with acute monoblastic leukemia (AML M5) at relapse in 1982 confirmed as human with IEF of AST, MDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. J. Minowada, Fujisaki Cell Center, Okayama, Japan
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
80-90% RPMI 1640 + 10-20% FBS maintain at 0.5-1.0 x 106 cells/ml; seed out at ca. 0.8-1.0 x 106 cells/ml; split 1:2 to 1:4 every 3-4 days; after thawing, culture should first be started with 20% FBS and best in 24-well-plates at 37C with 5% CO2 maximal density at about 1.0-1.5 x 106 cells/ml;
doubling time of ca. 40 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (near tetraploid (82-96) with hyperdiploid stemline; 47<2n>X, -X, +2m, del(10)(p12-p13), t(12;16)(q24;q11)) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (CD3- CD13- CD14- CD15+ CD19- CD33-) * reverse transcriptase (reverse transcriptase negative)
- Gann 1984;75:660-664 - PMID: 6593267
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.