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SPECIES: mouse, (C57BL/6xDBA/2)F1
ECACC catalog code: 85061103
Description: Mouse (C57BL/6xDBA/2)F1 leukaemia lymphocyte
Viruses: See Properties
Depositor: Prof H Harris/Dr R Sutherland, Sir William Dunn School of Pathology, Oxford, UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Differentiation studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% Horse Serum (HS). Maintain cultures between 3-9x100,000 cells/ml; using 0.25% trypsin, 5% CO2; 37C.
Derived from Friend leukaemia virus-induced leukaemia in BDF mice. Produces spleen colonies in vivo (of myelomonocytic, megakaryocytic, and erythropoietic origin). Will form colonies in soft agar in the presence of 10% CSA (colony stimulating activity factor).
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- Nature 1979;277:471 - PMID: 763330
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.