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SPECIES: cat, domestic
ECACC catalog code: 86093002
Susceptibility to viruses: canine parvovirus * feline calicivirus * feline herpes * feline immunodeficiency * feline panleukopenia * feline picornavirus * feline reovirus * rabies
Description: Cat kidney
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Products: Carcinoembryonic antigen (CEA) Applications: Virus studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:8 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C.
Karyotype: 2n = 38, hypodiploid, modal no 36
CRFK cells were isolated from the cortical portion of the kidneys of a 10 - 12 week-old normal female domestic cat. CRFK extensively used in feline virus research. Susceptible to herpesvirus and feline picornaviruses producing CPE. (BVD Ag negative)
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 94
- In Vitro 1973;9:176-185 - PMID: 4130570
- J Natl Cancer Inst 1972;41:55-60 - PMID: 4338781
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.