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TISSUE/ORGAN: endothelium, pulmonary artery
ECACC catalog code: 89050801
Description: Bovine pulmonary artery endothelium
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Enzymatic studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
Medium 199 + 2mM Glutamine + 20% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C. Growth rate decreases with passage.
Karyotype: 2n = 60
Isolated from normal young un-weaned calf. Also contains smooth muscle cells. Cultures exhibit angiotensin-converting enzyme activity and react with antibodies to human factor VIII and alpha 2 macroglobulin.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 207, IZSBS: BS CL19
- Tissue Cell 1978;10:535 - PMID: 83028
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.