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SPECIES: monkey, African green
DSMZ catalog code: ACC 60
Morphology: fibroblast-like cells growing as monolayers
TRANSFORMED BY: SV40
Description: African green monkey kidney derived from CV-1, a simian cell line (cercopithecus aethiops), by transformation with an origin-defective mutant of SV-40; cells were described to support the growth of SV-40 viruses; classified as risk category 1 according to the German Central Commission for Biological Safety (ZKBS) confirmed as of primate origin with IEF of LDH, MPI, NP, PEP B
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HIV-, HTLV-I/II-
Depositor: Dr. R. Riebe, Friedrich-Loeffler-Institute, Insel Riems, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% Dulbecco's MEM + 10% FBS split confluent culture 1:3 to 1:5 every 2-3 days using trypsin/EDTA; seed out at ca. 1 x 106 cells/80 cm 2 ; cells are growing slowly at 37C with 5% CO2 about 2-3 x 106 cells/80 cm 2;
doubling time of ca. 35-48 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (simian hypodiploid karyotype with 25% polyploidy; 57(53-58)<2n>) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile (identical to COS-1)) * isoenzymes (confirmed as of primate origin with IEF of LDH,MPI,NP,PEP B)
Availability in cell line catalogues: ATCC: CRL 1651, IZSBS: BS CL103, ECACC: 87021302, ICLC: ATL96005
- Cell 1981;23:175-182 - PMID: 6260373
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.