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SPECIES: human, Caucasian
TUMOR: carcinoma, large cell
ECACC catalog code: 92031919
Description: Human Caucasian lung large cell carcinoma
Viruses: See Properties
Depositor: Dr P Twentyman, UKCCCR, Lincolns Inn Fields, London, UK (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Studies on lung cancer
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 using 0.25% trypsin/EDTA; 5% CO2; 37C.
Karyotype: Very complex
Derived from the pleural effusion of a 62-year-old Caucasian male. The cells are tumorigenic in nude mice. This cell line has only been subject to preliminary quality control procedures. Authentication tests such as DNA fingerprinting will be performed only when this cell line is requested.
Research council deposit: Yes;
Price Code: B;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ICLC: HTL95012
- Br J Cancer 1985;52:495 - PMID: 2998422
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.