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SPECIES: human, Caucasian
TUMOR: melanoma, metastatic
DSMZ catalog code: ACC 216
Morphology: fibroblastic cells growing mainly as adherent monolayer and partly in suspension
Description: human melanoma derived in 1992 from right axillary lymph nodes of a 43-year-old Caucasian man with metastatic maligant melanoma confirmed as human with IEF of AST, MDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. G. E. Moore, Colorodo Oncology Foundation, Denver, Colorado, USA
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
80% RPMI 1640 + 20% FBS split confluent culture 1:4 to 1:10 every 4-7 days using trypsin/EDTA at 37C with 5% CO2 celll harvest of about 18 x 106 cells/175 cm 2;
doubling time of ca. 40-60 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 2.5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (human hypotriploid karyotype; 60<3n>X-Y, -1, +3, -4, -5, +7, -10, -11, -12, -13, -14, -15, -16, -17, -18, +20, +22, der(1)t(1;?)(q11;?), i(3q), idic(3)(3cen->p24::1q11->qter), der(7)t(7;?)(q33;?)x2, der(9)t(9;?)(p11;?) * cytogenetics 2 (the large idic(3) marker and i(3q) derived from an earlier translocation, t(1;3)(q11;p24), present in a sideline) * immunological analysis (cytokeratin- desmin- endothel- GFAP- neurofilament- vimentin+) * isoenzymes (confirmed as human with IEF of AST, MDH, NP) * reverse transcriptase (reverse transcriptase negative)
- Cancer Genet Cytogenet 1993;69:108-112 - PMID: 8402545
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.