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DSMZ catalog code: ACC 200
Morphology: cells grow loosely adherent in monolayers, some cells grow as round cells in clumps in suspension
Description: human breast carcinoma established from the pleural fluid of a 52-year-old man with breast carcinoma in 1991 confirmed as human with IEF of AST, MDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. G. E. Moore, Colorado Oncology Foundation, Denver, Colorado, USA
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
85% RPMI 1640 + 15% FBS split 1:2 to 1:3 every 2-4 days without use of trypsin/EDTA (cells are only loosely adherent); seed out at ca. 3-5 x 106 cells/80 cm 2 at 37C with 5% CO2 cell harvest of about 15-20 x 106 cells/80 cm 2;
doubling time of ca. 60-80 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (human highly rearranged hyperdiploid karyotype with 11% polyploidy; 51(48-52)<2n>X, -Y, +1, -2, -6, +7, -10, -13, -15, -19, -21, -22, +12mar, del(1)(p12), der(1)t(1;?)(p12;?), der(6)t(1;6)(q25;q12), der(7)t(7;?)(q32/34;?), der(8) (8;?)) * cytogenetics 2 ((q24;?), der(12)t(12;?)(p13;?), der(15)t(15;?)(p12;?), der(17)t(17;?)(p12;?); carries small bisatellited marker) * isoenzymes (confirmed as human wth IEF of AST, MDH, NP) * reverse transcriptase (reverse transcriptase negative)
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.