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DSMZ catalog code: ACC 198
Morphology: grows as clusters and single cells in suspension
Description: human ovarian adenocarcinoma established from the ascites fluid stemming from the colon metastasis of a 46-year-old white woman with ovarian adenocarcinoma in 1986 confirmed as human with IEF of AST, MDH
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. G. E. Moore, Colorado Oncology Foundation, Denver, Colorado, USA
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS maintain at 0.5-1.0 x 106 cells/ml; optimal split ratio of 1:2 to 1:3 every 2-3 days; seed out at ca. 0.2-0.3 x 106 cells/ml 5% CO2 (or may be grown in closed flasks), 37C saturation density at about 2.5 x 10 6 cells/ml;
doubling time of ca. 30-40 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 2-3 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (human hyperdiploid karyotype with 22% polyploidy; 51(48-53)<2n>XX, +1, +7, +8, +19, +20, i(1q), der(8)t(8;?)(q21;?); dm present in 16% cells; i(1q) is associated with ovarian carcinoma) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (cytokeratin-, desmin-, endothel-, GFAP-, neurofilament-, vimentin+ (100%)) * isoenzymes (confirmed as human with IEF of AST, MDH) * reverse transcriptase (reverse transcriptase negative)
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.