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DSMZ catalog code: ACC 196
Morphology: adherent, epitheloid cells growing as monolayers
Description: human lung adenocarcinoma established from the pleural fluid of a 57-year-old woman with adenocarcinoma of the lung in 1986 confirmed as human with IEF of AST, MDH
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. G. E. Moore, Colorado Oncology Foundation, Denver, Colorado, USA
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS split confluent cultures every 2-3 days using trypsin/EDTA; seed out about 1-2 x 106 cells/175 cm 2 at 37C with 5% CO2 cell harvest of about 12 x 106 cells/175 cm 2;
doubling time of ca. 38 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (human hypertriploid karyotype with 2% polyploidy; 70(62-72)<3n>XX, -X, +1, +3, -4, +6, +7, +7, +8, -9, -10, +12, -14, -16, -17, -17, +18, +20, der(2)t(2;10)(q27;q12), der(7)t(7;?)(p21;?)x2, i(10q), der(11)t(8;11)(q12;q14), idic(1 q),) * cytogenetics 2 (der(21)t(21;?)(p12;?)x2; a supernumerary small ring chromosome was present in 28% cells; the 8q12 breakpoint is associated with benign adenoma; corresponds to original karyotype with reinterpretation) * isoenzymes (confirmed as human with IEF of AST, MDH) * reverse transcriptase (reverse transcriptase negative)
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.