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DSMZ catalog code: ACC 21
Morphology: mostly round cells; about 80% are adherent cells; about 2-8% spindle-shaped cells; some giant cells
Description: human colon carcinoma established from the ascites fluid of a 71-year-old man with colon carcinoma in 1975 confirmed as human with IEF of AST, MDH
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. G. E. Moore, Colorado Oncology Foundation, Denver, Colorado, USA
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS split confluent cultures about 1:6 once a week using trypsin/EDTA at 37C with 5% CO2 about 7-10 x 106 cells/80 cm 2 ;;
doubling time of ca. 2-3 days;
frozen with 70% medium, 20% FBS, 10% DMSO at about 2.0.-2.5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (human hyperdiploid karyotype with 10% polyploidy; 72(66-77)XX, -Y, +1, +3, +3, +5, -6, +7, -8, +9, -10, -13, -18, +20, -21, +3 mar, i(1q), der(1)t(1;18)(q11;p11), der(3)t(3;?)(p21;?)x2, del(10)(q23.3), der(12)t(3;12)) * cytogenetics 2 (supernumerary centric fragment or ring present in 18% of cells) * DNA fingerprinting (unique DNA profile with (gtg)5 multilocus probe) * immunological analysis (cytokeratin+ desmin- endothel- GFAP- neurofilament- vimentin-) * isoenzymes (confirmed as human with IEF of AST, MDH) * reverse transcriptase (reverse transcriptase negative)
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.