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TISSUE/ORGAN: axilla lymph node
ECACC catalog code: 93051120
Description: Human melanoma
Viruses: See Properties
Depositor: Prof G Moore, Colorado Oncology Foundation, Denver General Hospital, Co 80204-4507, USA (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Tumourigenicity studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C.
Derived from a right axilla lymph node of a 45 year male with melanoma. The biopsy material was obtained post chemotherapy.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- Cancer Genet Cytogenet 1993;69:108-112 - PMID: 8402545
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.