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DSMZ catalog code: ACC 193
Morphology: epithelial-like cells growing as monolayer; some cells look like fibroblasts or neurons
Description: human melanoma established from the subcutaneous nodule of a 14-year-old boy with melanoma in 1990 confirmed as human with IEF of AST, MDH
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. G. E. Moore, Colorado Oncology Foundation, Denver, Colorado, USA
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90-95% RPMI 1640 + 5-10% FBS split confluent culture 1:4 to 1:5 two to three times per week using trypsin/EDTA; seed out at ca. 1-2 x 106 cells/80 cm 2 at 37C with 5% CO2 cell harvest of about 5 x 106 cells/80 cm 2;
doubling time of ca. 48 hours (with 5% FBS);
frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (hypotriploid karyotype with minor hypertriploid clone and 8% polyploidy; 64(50-69)<3n>X, -Y, -X/Y, -4, -5, +7, -8, -9, -10, -11, -12, +13, +13, +14, +15, -16, -19, +20, -21, -21, der(3)t(3;?)(p11;?), der(13q21q)x2, del(14)(q11/12) * cytogenetics 2 (der(15)t(15;?)(q26;?)x2; identitity confirmed by marker correspondence) * immunological analysis (cytokeratin-, desmin-, endothel-, GFAP- neurofilament-, vimentin++ (100%)) * isoenzymes (confirmed as human with IEF of AST, MDH) * reverse transcriptase (reverse transcriptase negative)
- Cancer Genet Cytogenet 1993;68:1002
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.