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TISSUE/ORGAN: lymphoblastoid B cell
DSMZ catalog code: ACC 206
Morphology: lymphoblast-like cells growing in suspension as single cells and in clumps
Description: human B-lymphoblastoid cells (from patient with adenocarcinoma) B-lymphoblastoid cell line established from the peripheral blood of a 73-year-old woman with metastatic adenocarcinoma in 1988 confirmed as human with IEF of AST, MDH
Viruses: ELISA: reverse transcriptase negative; PCR: EBV+, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. G. E. Moore, Colorado Oncology Foundation, Denver, Colorado, USA
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS maintain at 0.5-1.0 x 106 cells/ml at 37C with 5% CO2 (or may be grown in closed flasks) saturation density at about 2.0-2.5 x 10 6 cells/ml;
doubling time of ca. 20-30 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (hyperdiploid karyotype with 10% polyploidy; 47(45-50)<2n>XX, +12, der(5)t(3;5)(p21.3;p15); the 3p21.3 breakpoint is associated with benign adenoma) * immunological analysis (CD3- CD10- CD13- CD19+ (80%) CD20+ (95%) CD37+ (95%) HLA-DR+ (95%) cy/sIgM-cy/sIgG+ cy/s_+ cy/s_-) * isoenzymes (confirmed as human with IEF of AST, MDH) * reverse transcriptase (reverse transcriptase negative)
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.