This is the complete description of the cell line that you selected.
By clicking on a generic term (e.g., SPECIES) you will go to the corresponding index (e.g., index of species), while by clicking on a specific term (e.g., retinoblastoma) you will go to the list of corresponding cell lines (e.g., retionoblastoma cell lines).
ICLC catalog code: HTL95027
Continuous culture, grown as semiadherent
undifferentiated, round cells in suspension and loosely adherent cells in monolayer morphology
SPECIES: human (female, 55 years old)
DEPOSITOR: Obtained from ECACC
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: production of hormones: epinephrine, serotonin, norepinephrine, adrenocorticotropic, parathyroid hormone
Available in the following LABORATORY:
- IRCCS AOU San Martino -IST Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
RPMI 1640 + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:3-1:6: trypsin/EDTA is not necessary as cells are partly in suspension and partly only loosely adherent; seed at 1-3 x 104 cells/cm2; 37C, 5% CO2, mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as human with MD, MPI)
- Cancer Res 1979;39:4914-4924 - PMID: 498117
- Cancer Res 1993;53:5600-5604 - PMID: 8242610
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.