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SPECIES: mouse, Swiss albino
ECACC catalog code: 85022108
Susceptibility to viruses: herpes simplex * polyoma virus * Sendai * SV40 * vesic. stomatitis (Indiana)
Description: Mouse Swiss Albino embryo fibroblast
Viruses: See Properties
Depositor: Prof H Harris/Dr R Sutherland, Sir William Dunn School of Pathology, Oxford, UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Contact inhibition and virus studies: polyoma and SV40 viruses
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:10 to 1:20 i.e. seeding at 5x1,000 to 1x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C.
Karyotype: 2n = 40; hypertriploid
Evolved under conditions favouring retention of the property of contact inhibition of cell proliferation; they exhibit much more contact inhibition than diploid fibroblasts freshly plated in culture. Readily transformed by polyoma and SV40 viruses resulting in reduced sensitivity to contact inhibition.
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 92
- Arch Virol 1984;80:47 - PMID: 6200094
- J Cell Biol 1963;17:229-313
- J Cell Physiol 1979;100:139-146 - PMID: 313933
- Virology 1965;27:179-185 - PMID: 4284655
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.