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SPECIES: human, Caucasian
DSMZ catalog code: ACC 144
Morphology: undifferentiated, round cells in suspension and loosely adherent in monolayers
Description: human colon adenocarcinoma established from the tumor mass of a 55-year-old Caucasian woman with a moderately undifferentiated adenocarcinoma of the sigmoid colon in 1977; cells were described to produce several hormones, e.g. serotonin, epinephrine; cells express c-myc mRNA confirmed as human with IEF of AST, G6PD, MDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. H. Kirchner, Medical School of Hannover, Hannover, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS split confluent culture 1:2 to 1:3 every 24-48 hours (trypsin/EDTA is not necessary as cells are only loosely adherent) at 37C with 5% CO2 maximal density at about 7 x 106 cells/ml;
doubling time of ca. 38 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 106 cells/ampoule;
contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (hyperdiploid karyotype; 53(50-54)<2n>XX, -3, -3, +5, +7, +12, -13, -17, +18, +22, +6mar, t(1;5) (p11;q11), der(5)t(5;?)(p15;?), der(7)t(7;?)(q22;?), der(9)t(9;?)(p11;?), del(9)(p22), del(12)(p12), i(13q), der(14)t(14;?)() * cytogenetics 2 (der(18)t(18;?)(p11;?); variable numbers of supernumerary dmins were present in 90% of cells) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (cytokeratin+ desmin- endothel- GFAP- neurofilament- vimentin-) * isoenzymes (confirmed as human with IEF of AST, GPDH, MDH, NP)
Availability in cell line catalogues: ATCC: CCL 220
- Cancer Res 1979;39:4914-4924 - PMID: 498117
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.