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SPECIES: mouse, C57BL/ICRF
ECACC catalog code: 86082105
Description: Mouse C57BL/1CRF lung carcinoma
Viruses: See Properties
Depositor: Prof H Harris/Dr C Madin, Sir William Dunn School of Pathology, Oxford, UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Metastasis studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
Waymouth's MB 752/1 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Karyotype: Modal No 85
Derived from an alveologenic lung carcinoma from a C57BL/1CRF mouse. Can be maintained in a completely defined serum free medium, but the growth rate is much slower than in supplemented serum. Used in studies of lung metastases.
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
- Cancer Res 1976;36:1049-1055 - PMID: 1253168
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.