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TISSUE/ORGAN: peripheral blood
TUMOR: leukemia, T cell
DSMZ catalog code: ACC 7
Morphology: single, round cells in suspension
Description: human T cell leukemia established from the peripheral blood of a 36-year-old woman with chronic myeloid leukemia (CML) in blast crisis in 1987; cells were described to express T cell surface markers and to have a bcr-abl translocation (producing the p210 bcr-abl protein) confirmed as human with IEF of AST, MDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. K. Kuriyama, Nagasaki University School of Medicine, Nagasaki, Japan
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% Iscove's MDM or RPMI 1640 + 10% FBS maintain at 0.1-1.0 x 106 cells/ml; split 1:2 to 1:4 every 2-3 days at 37C with 5% CO2 maximal density at 2.5-3.0 x 106 cells/ml;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (near tetraploid karyotype with diploid sideline; 92<4n>XXXX, t(6;7)(q24;q35)x2, del(11)(q22.3)x2) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (CD3+ CD4+ CD5+ CD6+ CD7+ CD8+ CD13- CD19- TCR_beta-) * isoenzymes (confirmed as human with IEF of AST, MDH, NP) * reverse transcriptase (negative for reverse transcriptase, HTLV-I/II, HIV-1)
- Blood 1989;74:1381-1387 - PMID: 2788468
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.