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ECACC catalog code: 90031403
TRANSFORMED BY: murine sarcoma virus
Susceptibility to viruses: feline leukemia
Description: Cat kidney fibroblast, MSV transformed
Viruses: See Properties
Depositor: Dr O Jarrett, Department of Veterinary Pathology, University of Glasgow, UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus and cloning studies: FeLV, Feline endogenous retrovirus
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:4 to 1:5 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37C.
Susceptible to Feline leukaemia virus. There is the possibility of low level secretion of Feline endogenous retrovirus (RD 114). The cells should be handled under laboratory containment level 2.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- J Virol 1974;14:177-179 - PMID: 4365331
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.