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ECACC catalog code: 88021103
Morphology: Epithelial with occasional giant cells
Susceptibility to viruses: influenza A * influenza B * poliovirus 1 * vesic. stomatitis (Indiana)
Description: Human conjunctiva, HeLa characteristics
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Plaque assays, cloning, genetic and virus studies: swine influenza viruses
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
Medium 199 (HBSS) + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using EDTA or 0.25% trypsin; 5% CO2; 37C.
Karyotype: 2n = 46
Chang conjunctival cells adapted by Wong and Kilbourne, and designated as conjunctival 'D' cells. Susceptible to swine influenza viruses, supports production of plaque formation by variety of influenza virus subtypes. Has HeLa markers, exhibits type A G6PD This cell line was found to have a HeLa profile by DNA fingerprinting.
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 20.2
- In Vitro 1978;14:469-475 - PMID: 566722
- J Exp Med 1961;95:113
- Nature 1976;259:211-213 - PMID: 1250349
- Proc Soc Exp Biol Med 1954;87:440
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.