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SPECIES: hamster, Chinese
DSMZ catalog code: ACC 110
Morphology: adherent, fibroblastoid cells, may be induced to undergo additional differentiation
Susceptibility to viruses: arbovirus * Getah arbovirus * mengovirus * vesic. stomatitis (Indiana)
Description: Chinese hamster ovary cells subclone from parental CHO cell line that was initiated from an ovary biopsy of an adult Chinese hamster in 1957 confirmed as hamster with IEF of NP, PEP B
Viruses: ELISA: reverse transcriptase negative
Depositor: Dr. P. E. Bryant, University of St. Andrews, St. Andrews, Fife, UK
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% Ham's F12 + 10% FBS split confluent cultures 1:3 to 1:10 using trypsin/EDTA; seed out about 10 5 - 10 6 cells in an 80 cm 2 flask; cells grow up in 3-4 days to confluence at 37C with 5% CO2 about 10 x 106 cells/80 cm 2 or 3 x 10 6 cells/25 cm 2;
doubling time of ca. 24 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (near diploid with 12% polyploidy; 22(20-23); exhibits distinctive CHO karyology) * DNA fingerprinting (same Hinf I-(gtg)5 DNA profile as CHO-DHFR) * reverse transcriptase (reverse transcriptase negative)
Availability in cell line catalogues: ATCC: CCL 61, ECACC: 85051005, ICLC: ATL98003
- J Exp Med 1958;108:945
- Proc Natl Acad Sci U S A 1965;52:288 - PMID: 5244736
- Proc Natl Acad Sci U S A 1968;60:1275 - PMID: 5244736
- Virology 1965;27:11 - PMID: 4283990
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.