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SPECIES: mouse, Swiss 3T3
ECACC catalog code: 90092701
TRANSFORMED BY: Moloney-MSV
Description: Mouse Swiss 3T3 embryo, Moloney-MSV transformed
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Products: Growth factor Applications: Cell growth studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 5% Fetal Bovine Serum (FBS). Split confluent cultures 1:6 to 1:8 i.e. seeding at 1-2x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37C. Care is required during subculture as cells have a tendency to easily detach from the tissue culture flask.
The cells do not produce virus particles. In serum-free media the cells release a specific growth factor. This cell line is of Swiss 3T3 origin, transformed by the Moloney murine sarcoma virus. It is a non-producer of the virus particles. In serum-free media the line will release a growth factor which can be used to stimulate quiescent cells to grow in monolayer as well as in soft agar.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 1568
- Int J Cancer 1970;6:95 - PMID: 4319233
- Proc Natl Acad Sci U S A 1978;75:4001 - PMID: 211512
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.