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SPECIES: hamster, Chinese
DSMZ catalog code: ACC 126
Morphology: epithelial-like adherent monolayer
Description: Chinese hamster ovary cells subclone of the parental line CHO which was derived from the ovary of an adult Chinese hamster in 1957; the cells are dehydrofolate reductase negative (dhfr-; Dukx cells); patented cell line confirmed as hamster with IEF of AST, NP
Viruses: ELISA: reverse transcriptase negative
Depositor: Dr. H. Hauser, GBF, Braunschweig, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% alpha-MEM + 10% FBS + 2 mM L-glutamine split confluent cultures 1:3 or higher every 2-4 days using trypsin/EDTA; maximum density at 90 x 106 cells/175 cm 2 ; seed out at ca. 0.1-1.0 x 106 cells/80 cm 2 in 10 ml medium at 37C with 5% CO2 about 10 x 106 cells/80 cm 2 or 40-70 x 106 cells/175 cm 2;
doubling time of ca. 20-24 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile)
Availability in cell line catalogues: ATCC: CRL 9096
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.