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SPECIES: hamster, Chinese
ECACC catalog code: 94060607
Description: Hamster Chinese ovary
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: This material is cited in a US and/or other Patent and may not be used to infringe patent claims.
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
IDMEM + 2mM Glutamine + 0.1mM Hypoxanthine + 0.01mM Thymidine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:4 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C A full description of the recommended subculture methods and documentation of the cell bank production is provided with the cells.
Karyotype: Modal No 20
The cells are deficient in dihydrofolate reductase. They are available free of charge to organisations wishing to produce biological products e.g. vaccines, in accordance with the recommendation of the Expert Committee on Biological Standardisation (Technical Report Series No. 786, WHO Geneva 1989 page 12: WHO Cells banks of continuous cell lines for the production of biologicals).
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 9096
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.