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ICLC catalog code: HTL05008
Continuous culture, grown as suspension
large, round, single cells morphology
SPECIES: human (male, 1 years old)
TISSUE/ORGAN: peripheral blood
TUMOR: leukemia, acute monocytic
DEPOSITOR: Dr A. De Lerma, Clinical and Biological Science Dept, Faculty of Medicine, Insubria University, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: Low constitutive expression of HLA-II; high expression of HLA-II after treatment with IFNgamma.
Available in the following LABORATORY:
- IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
RPMI 1640 + 10% FBS + 2mM L-Glutamine; Freezing medium: 90% FBS + 10% DMSO; Maintain cultures between 2-9x105 cells/ml; 37C, 5% CO2, mycoplasma negative, HOECHST and PCR
Validated by: identification confirmed by Short Tandem Repeat (STR) profile (Amelogenin: X,Y; vWA: 16; FGA: 24,25; TH01: 8,9.3; D18S51: 13,14; D21S11: 30,31.2; D8S1179: 10,14) * Multiplex PCR (tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers)
Molecular authentication: Amelogenin: X,Y ; D18S51: 13,14 ; D21S11: 30,31.2 ; D8S1179: 10,14 ; FGA: 24,25 ; TH01: 8,9.3 ; VWA: 16
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.