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ICLC catalog code: HTL05004
Continuous culture, grown as suspension
lymphoblast, single and in small clusters growth morphology
SPECIES: human (female)
TISSUE/ORGAN: lymphocyte, B
PATHOLOGY: paroxysmal nocturnal hemoglobinuria+311770 (OMIM record)
TRANSFORMED BY: Epstein-Barr
DEPOSITOR: Dr R. Notaro, Human Genetic Dept IST, Genoa, Italy
Cell line available for distribution. For non-commercial investigative use only
Available in the following LABORATORY:
- IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
RPMI 1640 + 4 mML-Glutamine + 20% FBS ; Freezing medium: 90% FBS + 10% DMSO; maintain cultures between 350-400 x 10^3 cells/ml; duplication time 48-60 h; 37C, 5% CO2, mycoplasma negative, HOECHST and PCR
Handle under Biosafety Level 2 containment
Validated by: Multiplex PCR (tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers)
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.