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ICLC catalog code: HTL05006
Continuous culture, grown as suspension
lymphoblast-like, cells growing single and in clusters morphology
SPECIES: human, Caucasian (male, 53 years old)
TISSUE/ORGAN: lymphocyte, T
DEPOSITOR: Dr. A. De Maria, Internal Medicine Dept (DIMI), Genoa, Italy
Cell line available for distribution. For non-commercial investigative use only
Available in the following LABORATORY:
- IRCCS AOU San Martino -IST Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
RPMI 1640 + 10% FBS + Antibiotics + 2mM L-Glutamine; Freezing medium: 90% FBS + 10% DMSO; split 1:10 every 2 days; 37C, 5% CO2, mycoplasma negative, HOECHST and PCR
Thaw the cells with medium supplemented with FBS 20%
Validated by: Multiplex PCR (tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers)
Availability in cell line catalogues: ECACC: 85050301
- AIDS Res Hum Retroviruses 1989; 5(3):253-5 - PMID: 2567177
- Blood 1980; 55:409-417 - PMID: 6244013
- J Natl Cancer Inst 1992; 84:1922-1926 - PMID: 1460674
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.