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ICLC catalog code: EBV03009
Continuous culture, grown as suspension
lymphoblast, cells growing as single cells or in clusters, some adherent morphology
SPECIES: human (male, 77 year old)
TISSUE/ORGAN: lymphocyte, B
TRANSFORMED BY: Epstein-Barr
DEPOSITOR: Cell line established at ICLC cell bank. Sample provided by the Rome Twin Register
Cell line available for distribution. For non-commercial investigative use only
Available in the following LABORATORY:
- IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
RPMI 1640 + 20% FBS + 2mM L-Glutamine; Freezing medium: 90% FBS + 10% DMSO; Maintain cultures between 3-9 x 100.000 cells/ml; split 1:4-1:8; 37C, 5% CO2 Passage: 4, mycoplasma negative, HOECHST and PCR
Handle as Biosafety Level 2 containment
Dizygous twin of IST-EBV-TW5B
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.