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ICLC catalog code: HTL03004
Continuous culture, grown as suspension
round cells, erythromegakaryocytic morphology
SPECIES: human (male, 14 year old)
TISSUE/ORGAN: bone marrow
DEPOSITOR: Prof. G.P. Bagnara, Institute of Histology and Embryology, University of Bologna, Italy
Cell line available for distribution upon consent of the depositor. For non-commercial investigative use only
PROPERTIES: Expression of both c-mpl and erythropoietin receptor (Epo-R). Spontaneous B1647 cell proliferation is significantly potentiated by thrombopoietin (TPO)
Available in the following LABORATORY:
- IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
Iscove's modified Dulbecco's medium + 10% FBS + 2mM L-Glutamine ; Freezing medium: Culture medium + 70% FBS + 10% DMSO; Split 1:3-1:6; 37C, 5% CO2 Passage: >50, mycoplasma negative, HOECHST and PCR
Validated by: Multiplex PCR (tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers)
- Br J Haematol 1997;98:549-559 - PMID: 9332307
- Haematologica 2002;87:1242-1247 - PMID: 12495897
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.