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ICLC catalog code: HTL03003
Continuous culture, grown as monolayer
TISSUE/ORGAN: kidney, embryonic
TRANSFORMED BY: adenovirus 5
DEPOSITOR: Obtained from ECACC
Cell line available for distribution. For non-commercial investigative use only
Available in the following LABORATORY:
- IRCCS AOU San Martino -IST Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
DMEM + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Freezing medium: 90% FBS + 10% DMSO; Split cultures 1:3-1:6 using trypsin/EDTA; 37°C, 5% CO2. The viability may be low after thawing and the cells may take up to 7 days to attach. The cells should be frozen at not less than 8 x 10^6 /ampoule and thawed with 20% FBS, mycoplasma negative, HOECHST and PCR
Handle as Biosafety Level 2 containment
Validated by: Multiplex PCR (tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers)
Availability in cell line catalogues: ECACC: 85120602, DSMZ: ACC 305
- J Gen Virol 1977;36:59-74 - PMID: 886304
- Virology 1977;77:319-329 - PMID: 841862
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.