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ICLC catalog code: HTL03002
Continuous culture, grown as suspension
lymphoblast, round, large and single cells. Some giant cells morphology
SPECIES: human, Caucasian (male, 30 year old)
DEPOSITOR: Obtained from Dr A. De Lerma Barbaro, Clinical and Biological Sciences, University of Insubria, Varese, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: Used in the study of erythroid cell differentiation and globin gene expression
Available in the following LABORATORY:
- IRCCS AOU San Martino -IST Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
RPMI 1640 + 10% FBS + 2mM L-Glutamine; Freezing medium: 90% FBS + 10% DMSO; Maintain cultures between 2-9 x 10^5 cells/ml; split 1:3-1:10; 37°C, 5% CO2, mycoplasma negative, HOECHST and PCR
Some cells weakly adherent
Validated by: Multiplex PCR (tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers)
Availability in cell line catalogues: ECACC: 92111706
- Science 1982;216:1233-1235 - PMID: 6177045
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.