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SPECIES: mouse, 129
ECACC catalog code: 95011018
Morphology: Small, tightly packed
Description: Mouse embryonic stem cell
Viruses: See Properties
Depositor: Dr A Smith, AFRC Centre for Genome Research, Edinburgh, UK (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Gene targeting, Gene trapping, in vitro differentiation
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
GMEM + 2mM Glutamine + 100 units/ml DIA/LIF + 0.05mM 2-Mercaptoethanol (2ME) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:10 i.e. seeding at 4x1000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
The germ-line competent cell line CGR8 was established from the inner cell mass of a 3.5 day male pre-implantation mouse embryo (Mus musculus, strain 129). These pluripotent cells retain the ability to participate in normal embryonic development. Differentiation of CGR8 cells is inhibited by the pleiotropic cytokine Differentiation Inhibiting Activity (DIA) which is identical to Leukaemia Inhibiting Factor (LIF). Addition of DIA/LIF allows culture of CGR8 without the use of feeder layers.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- Development 1990;110:1341 - PMID: 2129226
- J Tiss Cult Meth 1991;13:89
- Proc Natl Acad Sci U S A 1994;91(10):4303-4307
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.