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ICLC catalog code: ATL01002
Continuous culture, grown as monolayer
SPECIES: monkey, African green
Original line: CV-1
TRANSFORMED BY: SV40
DEPOSITOR: Dr A. De Lerma Barbaro, Clinical and Biological Sciences, University of Insubria, Varese, Italy.
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: This line contains T antigen. Suitable for transfection by vectors requiring expression of SV40 T antigen
Available in the following LABORATORY:
- IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
DMEM + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; split confluent cultures 1:4-1:10 using trypsin/EDTA; 37C, 5% CO2, mycoplasma negative, HOECHST and PCR
Validated by: PCR analysis (confirmed as African green monkey with cytochrome c oxidase subunit I (cox I) primers)
Availability in cell line catalogues: IZSBS: BS CL95, ECACC: 88031701, DSMZ: ACC 63
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.