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ICLC catalog code: HTL01015
Continuous culture, grown as suspension
round, multiformed cells, some giant cells morphology
SPECIES: human (male, 64 year old)
TISSUE/ORGAN: peripheral blood
TUMOR: leukemia, acute monocytic
DEPOSITOR: Dr A. De Lerma Barbaro, Clinical and Biological Sciences, University of Insubria, Varese, Italy.
Cell line available for distribution. For non-commercial investigative use only
Available in the following LABORATORY:
- IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
RPMI 1640 + 10% FBS + 2mM L-Glutamine + 1mM Na pyruvate + 1% non-essential amino acids + 9 ug/ml bovine insulin ; Freezing medium: 90% FBS + 10% DMSO; maintain cultures between 3x10^5 and 1x10^6 cells/ml; split 1:3-1:6; 37C, 5% CO2, mycoplasma contamination eliminated with ciprofloxacin; negative Hoechst and PCR
Validated by: HLA-DP beta (confirmed as human by PCR)
Availability in cell line catalogues: DSMZ: ACC 124
- Int J Cancer 1988;41:456-461 - PMID: 3162233
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.