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SPECIES: human, Caucasian
ECACC catalog code: 90021502
Description: Human Caucasian astrocytoma
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: HLA profiles
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Established from Grade IV astrocytoma from 68-year old Caucasian female. Cells exhibit strong perinuclear acid and phosphatase activity, glial fibrillary acidic protein present in 70-80% cells. HLA-DR present on approx. 20-30% cells after 48 hours culture.
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 1718, IZSBS: BS TCL 125
- Fed Proc 1984;43:781
- In Vitro 1983;19:275
- J Neuroimmunol 1985;10:151 - PMID: 3877740
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.