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SPECIES: rat, Wistar
ECACC catalog code: 93070901
Description: Rat Wistar liver, normal
Viruses: See Properties
Depositor: Prof P A Riley, University College London, Medical School, London, UK
No restrictions. Patent: None Specified By Depositor
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 20mM Hepes + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:1000 i.e. seeding at 5x1,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
A subclone of the line RLC which was derived from the normal liver cells of a male Wistar rat.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- Differentiation 1978;10:159
- Science 1970;170:859 - PMID: 4394388
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.