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SPECIES: mouse, Swiss albino
DSMZ catalog code: ACC 173
Morphology: fibroblasts growing adherently as monolayer with contact inhibition
Description: Swiss albino mouse fibroblasts established from disaggregated Swiss albino mouse embryos in 1962; cells are used for transfection studies with DNA viruses and as assay system for transformation studies; cells have a high sensitivity to contact inhibition (= ATCC CCL 92) confirmed as mouse with IEF of AST, LDH, PEP B
Viruses: ELISA: reverse transcriptase negative
Depositor: Dr. J. Wehland, GBF, Braunschweig, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90-95% Dulbecco's MEM + 5-10% FBS split confluent culture at a ratio of up to 1:8 every 2 to 4 days using trypsin/EDTA; after trypsination cells might remain in aggregates that are difficult to disperse; upon reaching confluence cells stop to grow; cells grow fast at low titer; seed out at ca. 1-2 x 106 cells/175 cm 2 at 37C with 5% CO2 cell harvest of about 5-8 x 106 cells/175 cm 2;
doubling time of ca. 40 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at 1 x 106 cells/ampoule;
mycoplasma negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (murine hypertriploid (near tetraploid sideline present) karyotype with 13% polyploidy; 68(58-73)<3n>) * isoenzymes (confirmed as mouse with IEF of AST, LDH, PEP B)
Availability in cell line catalogues: ICLC: ATL95002, IZSBS: BS CL 82
- J Cell Biol 1963;17:299-313
- Virology 1965;27:179-185 - PMID: 4284655
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.