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SPECIES: fish - goldfish
ECACC catalog code: 89072611
Susceptibility to viruses: grunt fin agent virus * infectious pancreatic necrosis
Description: Goldfish fin tissue
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Growth factors and virus studies: Infectious pancreatic necrotic virus
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
BME (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:4 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 25C.
Karyotype: Hypodiploid, Modal No 97.
The cells support the growth of grunt fin agent with CPE at 20C and 25C. Also susceptible to infectious pancreatic necrosis virus of trout at 28C.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 71, IZSBS: BS CL12
- Ann N Y Acad Sci 1964;126:344
- In Vitro 1980;16:168 - PMID: 6767657
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.