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SPECIES: human, Caucasian
DSMZ catalog code: ACC 245
Morphology: adherent, epithelial-like cells growing in uniform monolayers
Description: human pancreas adenocarcinoma established from the tumor of a 56-year-old Caucasian man with pancreas adenocarcinoma in 1975 confirmed as human with IEF of AST, MDH, PEP B
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: obtained from DKFZ Tumorbank, Heidelberg, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
85% RPMI 1640 + 15% FBS split semi-confluent (2/3 to 3/4 confluency) culture 1:10 every 7 days using trypsin/EDTA (for 10-20 min); seed out at ca. 1-2 x 106 cells/80 cm 2 , adherence may require several hours or overnight at 37C with 5% CO2 cell harvest of about 20 x 106 cells/175 cm 2;
doubling time of about 50-70 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (hypertriploid karyotype with 6% polyploidy; 68- 73<3n>XX, -Y, +1, +1, +4, +5, +7, +11, -12, -14, -17, -18, -22, +3mar; del(1)(p11p33)x2, add(11)(p11), der(11)t(?8;11)(q12;p11)x2, i(14q)x2, add(21)(p11)x2, 1-2DM(24%); sdl with +1,) * cytogenetics 2 (i(1q)x2, del(1)(q11)) * DNA fingerprinting (unique DNA profile with (gtg)5 multilocus probe) * immunological analysis (cytokeratin+, desmin-, endothel-, GFAP-, neurofilament-, vimentin-) * isoenzymes (confirmed as human with IEF of AST, MDH, PEP B) * reverse transcriptase (reverse transcriptase negative)
Availability in cell line catalogues: ATCC: HTB 80
- Cancer Res 1986;46:5810-5815 - PMID: 3019537
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.