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SPECIES: human, Caucasian
DSMZ catalog code: ACC 244
Morphology: adherent fibroblastic semi-confluent monolayer forming loosely adherent foci
Description: human pancreas adenocarcinoma established from the liver metastasis of a pancreatic ductal adenocarcinoma in a 40-year-old Caucasian man in 1974; described as growing in nude mice forming metastases and as resistant to 5-FU confirmed as human with IEF of AST, MDH
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HIV-, HTLV-I/II-
Depositor: obtained from DKFZ Tumorbank, Heidelberg, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
80% RPMI 1640 + 20% FBS split 1:2 to 1:4 every 6-10 days using trypsin/EDTA; initial growth after recovery is slow; seed out at ca. 1-2 x 106 cells/80 cm 2 at 37C with 5% CO2 cell harvest of about 1.5 x 105 cells/cm 2;
doubling time of about 50-100 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (apparently human karyotype) * DNA fingerprinting (unique DNA profile with (gtg)5 multilocus probe) * immunological analysis (cytokeratin+, desmin-, endothel-, GFAP-, neurofilament-, vimentin-) * isoenzymes (confirmed as human with IEF of AST, MDH) * reverse transcriptase (reverse transcriptase not detected)
Availability in cell line catalogues: ATCC: HTB 79
- Cancer Res 1986;46:5810-5815 - PMID: 3019537
- J Natl Cancer Inst 1977;58:209-214 - PMID: 833871
- J Natl Cancer Inst 1977;59:221-226 - PMID: 327080
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.