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TUMOR: carcinoma, squamous cell
DSMZ catalog code: ACC 187
Morphology: adherent, epithelial-like cells growing as monolayer
Description: human vulva squamous cell carcinoma established from the primary lesion resected from a 76-year-old woman with bulky, moderately differentiated squamous cell carcinoma of the vulva in 1984; cells were reported to express an elevated number of high affinity EGF receptors, to carry gene amplification at 11q13 and to be tumorigenic in nude mice confirmed as human with IEF of AST, MDH
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. J. Gioanni, Centre Antoine Lacassagne, Nice, France
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
80% Dulbecco's MEM + 20% FBS + 2 mM L-glutamine + 1 mM sodium pyruvate + 0.5 nM hydrocortisone + 1 ęg/100 ml EGF cells are difficult to culture! split confluent culture 1:2 to 1:4 every 5-10 days using trypsin/EDTA (cells are sensitive to over-trypsination) at 37C with 5% CO2 cell harvest of about 0.8 x 105 cells/cm 2;
doubling time of about 50-70 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 0.75-1.5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (apparently human karyotype) * DNA fingerprinting (unique DNA profile with (gtg)5 multilocus probe) * immunological analysis (cytokeratin+ (100%) desmin- endothel- GFAP- neurofilament- vimentin-) * isoenzymes (confirmed as human with IEF of AST, MDH) * reverse transcriptase (reverse transcriptase not detected)
- Int J Oncol 1993;3:293-297
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.