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DSMZ catalog code: ACC 142
Morphology: epithelial-like, adherent in monolayers
Description: human kidney carcinoma established from the kidney carcinoma (clear cell carcinoma) of a 49-year-old man; tumorigenic in mice confirmed as human with IEF of G6PD, MDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. H. Kirchner, Medical School of Hannover, Hannover, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% McCoy's 5A + 10% FBS split confluent culture at 1:3 to 1:5 using trypsin/EDTA; upon thawing grow immediately in a 80 cm 2 flask; seed out at ca. 1-2 x 106 cells/80 cm 2 at 37C with 5% CO2 about 3-5 x 106 cells/80 cm 2;
doubling time of ca. 48 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (flat-moded penta-/hexa-ploid karyotype; 112-149<6n>XXX, -Y, -Y, -Y, rearrangements include add(1)(p22), i(3p), add(3)(p14), der(7)t(7;7)(q34;p14),i(8q), i(10q), add(11)(p11),) * cytogenetics 2 (large isodie marker x2; does not correspond with published karyotype) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (CD31-, cytokeratin+ desmin- GFAP- neurofilament- vimentin+) * isoenzymes (confirmed as human with IEF of G6PD, MDH, NP) * reverse transcriptase (negative)
Availability in cell line catalogues: ATCC: HTB 46, IZSBS: BS TCL 108
- Human Tumor Cells in Vitro (Plenum Press N Y) 1975;115-159
- J Natl Cancer Inst 1977;58:209-214 - PMID: 833871
- Natl Cancer Inst Monogr 1978;49:5-19 - PMID: 571047
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.