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SPECIES: human, Caucasian
DSMZ catalog code: ACC 169
Morphology: epithelial adherent cells; after splitting cells start to grow in colonies
Description: human colon adenocarcinoma established from the primary colon tumor (adenocarcinoma) of a 72-year-old Caucasian man in 1974 confirmed as human with IEF of MDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Prof. A. Bacher, Technical University of Munich, Munich, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
80% MEM (with Earle's salts) + 20% FBS + 1x non-essential amino acids cells grow slowly; split confluent culture 1:6 to 1:10 every 5-7 days using trypsin/EDTA or, if necessary, a cell scraper; after thawing cells might start slowly to take up growth; seed out at ca. 1 x 106 cells/80 cm 2 in 10 ml medium at 37C with 5% CO2 about 3-6 x 106 cells/80 cm 2 or ca. 1.0 x 105 cells/cm 2;
doubling time of ca. 80 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 106 cells/ampoule;
contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in microbiologcal culture, RNA hybridization, PCR assay;
Validated by: DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (cytokeratin+ desmin- endothel- GFAP- neurofilament- vimentin-) * isoenzymes (confirmed as human with IEF of MDH, NP) * reverse transcriptase (reverse transcriptase negative)
Availability in cell line catalogues: ATCC: HTB 37, ECACC: 86010202, ICLC: HTL97023, IZSBS: BS TCL 87
- Biochimie 1986;68:1035-1040 - PMID: 3096381
- Biol Cell 1983;47:323-330
- Exp Cell Res 1992;200:97-104 - PMID: 1563496
- J Natl Cancer Inst 1977;58:209-214 - PMID: 833871
- J Natl Cancer Inst 1977;59:221-226 - PMID: 327080
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.