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TUMOR: lymphoma, Burkitt
DSMZ catalog code: ACC 73
Morphology: single cells in suspension
Description: human Burkitt's lymphoma established from the ascites fluid of a patient with American-type Burkitt's lymphoma; cells were described to be EBNA-negative, to carry the (8;14) translocation and to express bcl-2 and c-myc mRNA confirmed as human with IEF of AST, MDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. H. G. Drexler, DSMZ, Braunschweig, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS maintain at 0.5-2.0 x 106 cells/ml, split 1:2 to 1:3; seed out at 0.5 x 106 cells/ml at 37C with 5% CO2 saturation density of over 2 x 106 cells/ml;
doubling time of about 35 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (near diploid with about 3% triploidy; a supernumerary dmin was present in about 25% metaphases; 46(45-48)<2n>X/XY, dup(1)(q21q32), dup(7)(q12q22), t(8;14)(q24;q32); additional rearrangements were present in a sideline, viz.t(6;1 )(p2) * cytogenetics 2 (and del(11)(p11)) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (CD3- CD10+ CD13- CD19+ CD37+ HLA-DR+ sIgM+) * isoenzymes (confirmed as human with IEF of AST, MDH, NP)
Availability in cell line catalogues: ATCC: CRL 1648
- J Immunol 1982;129:1336-1342 - PMID: 6286763
- J Natl Cancer Inst 1980;64:477-483 - PMID: 6243722
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.