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ECACC catalog code: 85081901
Transformed by: Chemical Agent Back To Chemical Agents' WebPage
Description: Rat nervous tissue oligodendroglioma
Viruses: See Properties
Depositor: Dr K Fields, Albert Einstein College of Medicine, Bronx, New York, USA (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Neurobiological studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C.
33B is a N-Ethyl-N-nitrosourea-induced neural tumour cell line derived from 17th in vivo passage of TR33B. The cell line is positive for rat neural antigen-1 (Ran-1) and also reacts with a marker for normal Schwann cells, the monoclonal antibody 217c.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- Neuroscience 1985;15:877 - PMID: 4069357
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.